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BioDataFit 1.02: Data Fit For Biologists Order Home

Interested in high throughput datafiting?
Click here BioDataFit is a data fitting program specifically designed for biologists. It can be used to model dose-response, ligand-binding, enzyme kinetics, and growth inhibition. Emphasis is given to the four-parameter model or sigmoidal model, which is frequently used to calculate EC50 (IC50, DC50, or GI50) values in dose-response experiments such as drug screening and inhibition assays.


Model	Application

Four Parameter Sigmoidal	Dose-response, drug screening, ligand binding, allosteric enzyme activity, and inhibition assays.

a x / ( x + b )	Enzyme kinetics, V_max, and K_M.

Linear	Standard curves.

a exp( -b x ) + c	Activity decay.

Usage To start, input your data in 2 (x and y) or 3 columns (x, y, and standard deviation of subpopulation of y). Chose a suitable model and click on "Build Model." Refine the model by removing outliers. To remove an outlier, check the "Mark Outlier" checkbox and then click on the outlier data point.
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BioDataFit Online runs on Java-enabled browsers. PC: Internet Explorer 5.5 or higher and Netscape 4.08 or higher. Mac: Internet Explorer 5.0, IE5.1.4 or higher (IE5.1 will not work because of a bug.)

The Electronic Protocol Book Table of contents

An electronic protocol book with 500 protocols and 100 recipes. A great quick and practical reference for bench scientists as well as for new students. (Available for both PC and Mac.) Get a copy

Glossary IC Inhibitory concentration. IC50 --- the concentration at which growth or activity is inhibited by 50%. Applies to ligand and growth inhibition.
ED or EC Effective dose (or concentration).
LogIC or LogEC IC or EC in log10 scale. Log10 scale is frequently used when x values are a serial dilution. Better estimate of the standard error is obtained when log10 scale is used.
LD Lethal dose. LD50 --- the dose at which 50% of test subjects die.
GI Growth inhibition. GI50 --- the drug concentration giving a 50% reduction in the net protein increase.
LC50 for drugs with cytotoxic effect, is the concentration of drug where 50% of cells die (a 50% reduction in the measured protein at the end of the drug treatment as compared to that at the begining).
TGI Total growth inhibition.
V_max Maximum reaction rate.
K_M Michaelis-Menten Constant. Michaelis-Menten equation for enzyme kinetics:
V₀ = V_Max [S] / ( K_M + [S] ).

High-throughput BioDataFit
BioDataFit HTP 1.2
Supports Wins/NT, Mac OSX, Linus, and Unix

Designed for high-throughput drug screens.

IC50 calculation for high-throughput inhibition assays.

Km or Vmax calculation for high-throughput enzyme kinetics assays.

EC50 calculation for high-throughput ligand-binding assays.

High-throughput linear-response assays.

Half-life calculation for high-throughput activity decay assays.

Automatically removes outliers for best results.

Interfaces for integrating to database and web-servers.

Command line for calling by scripts.

Save fitting curves as JPEG files.

Java application supports most systems.

Free tech support.

Customization to meet your needs.

Click here for more info.

What is a USB Flash Disk?
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