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Recipes

5-Fluoroanthranilic Acid (5-FAA 10%)
AEBSF (25 uM)
AHC medium
ATP (adenosine triphosphate 10 mM)
Acetic alcoho fixation
Aceto-orcein staining solution (1%)
Acetocarmine staining solution (1%)
Acetyl CoA
Acid alcohol (1%)
Acrylamide/Bisacrylamide (19:1, 40%)
Acrylamide/Bisacrylamide (37.5:1, 30%)
Actinomycli D (10 mg/ml)
Adenine (1%)
Agarose gel loading dye (10X)
Alkaline lysis solution (NaOH/SDS)
Amido black (methanol-based)
Amido black (water-based)
Ammonium acetate (5M)
Ammonium acetate (9.5M)
Ammonium acetate (NH4Ac 8.0M)
Ammonium persulfate (10%)
Ampicillin (50 mg/ml)
Anisomycin (10 mg/ml)
Aprotinin (10 mg/ml)
Attophos buffer (pH 9.5)
BME (beta-mercaptoethanl 0.5M)
BME (beta-mercaptoethanl 1M)
BSA (1mg/ml)
Betaine (5 M)
Blasticidin S (10 mg/ml)
Bleach (50%)
Bleach/Tween
Bouin fixation
Brij Buffer I
Brij Buffer II
Bromcresol Purple (4%)
Bromthymol blue pH indicator I (0.1%)
Bromthymol blue pH indicator II (0.1%)
Calcium chloride (CaCl2)
Carbenicillin (100 mg/ml)
Carnoy fixation
Caryon's fixing solution I
Caryon's fixing solution II
Chloramphenicol (25 mg/ml)
Citric acid (1M)
Coomassie Blue Stain Solution (ethanol)
Coomassie Blue Stain Solution (methanol)
Cresol Red (50 mM)
Cycloheximide (10 mg/ml)
D-Cycloserine (10 mg/ml)
DAPI (1 mg/ml)
DAPI (5 mg/ml)
DEPC treated water
DTT (Dithiothreitol 1M)
Denaturing Solution
Denhart Solution (100X)
Destain solution I (protein gel)
Destain solution II (protein gel)
Destain solution III (protein gel)
Dry milk blocking buffer
EDTA (0.5M pH8.0)
Eosin Y counterstain (1%)
Erythromycin (25 mg/ml)
EtBr (ethidium bromide 5 mg/ml)
Ethonal (70%)
Farmer's fixing solution
Formalin (10% neutral buffered)
Formalin (10% unbuffered)
Formalin (20% neutral buffered)
Formalin (20% unbuffered)
Galactose (30%)
Gelatin (2%)
Geneticin (200 mg/ml)
Gentamycin (10 mg/ml)
Glacial acetic acid (1M)
Glucose (1M)
Glucose (20%)
Glucose (40%)
Glucose minimal plates
Glutathione (100 mM)
Glycerol (50%)
Glycerol (Sterile)
Guanidine (6M pH6.4)
HEPES (1M pH7.5)
Helly fixation
Hydrochloric Acid (HCl 1M)
Hydroxyurea (100 mg/ml)
Hydroxyurea (250 mM)
Hygromycin B (100 mg/ml)
IPTG (20mg/ml)
IPTG (isopropyl b-D-thiogalactopyranoside 25 mg/ml)
Imidazol (2M)
Isocitrate (1M)
KCl (1M)
Kanamycin (25mg/ml)
Kasugamycin (200 mg/ml)
LB low salt medium
LB low salt plates
LB medium
LB plates
Lambda plates
Lambda top agar
Leupetin (10 mg/ml)
Lithium acetate (1 M)
Lithium chloride (LiCl 1 M)
M9 medium
M9 salts (10X)
MOPS (10X)
MOPS (1M)
Magnesium chloride (MgCl2 1M)
Magnesium sulfate (MgSO4 1M)
Malachite green stain (5%)
Manganese chloride (MnCl2 1M)
Mercuric chloride (40 mg/ml)
Michel fixation
NYZ agar
NYZ top agarose
NZY Broth
Nalidixic acid (100 mg/ml)
Neomycin (25 mg/ml)
Neutralizing Solution
P1 (15 mM Tris pH8 10mM EDTA plasmid prep)
P2 (0.2N NaOH 1% SDS plasmid prep)
P3 (3M KOAc pH 5.5 plasmid prep)
PEG (40%)
PEG/NaCl
PK Buffer (5X)
Phenol (TE-saturated)
Phenol/chloroform/isoamyl alcohol (25:25:1)
Phleomycin (20 mg/ml)
Plasmocin (25 mg/ml)
Polyacrylamide Gel loading dye
Potassium acetate (7.5M)
Propidium iodide (5 mg/ml)
Propionic acid-ethanol fixing solution
Proteinase K (10mg/ml)
Puromycin (10 mg/ml)
Raffinose (20%)
Retinoic acid (10 mM)
Rifampicin (200 mg/ml)
SM
SOB medium
SOC medium
SSC (20X)
SSPE (20X)
Salmon sperm DNA (10 mg/ml)
Sarkosyl (10%)
Sobitol (1 M)
Sobitol (2.5 M)
Sodium Acetate (3M pH5.2)
Sodium Acetate (NaAc 2M)
Sodium Acetate (NaAc 3M pH4.8)
Sodium chloride (5M)
Sodium chloride (NaCl 3M)
Sodium citrate (Na citrate 1M)
Sodium hydroxide (NaOH 10N)
Sodium hydroxide (NaOH 1N)
Sodium phosphate (pH6.7)
Spectinomycin (20 mg/ml)
Spermidine (0.1M)
Streptomycin (50 mg/ml)
Sulfuric Acid (H2SO4 1 M)
Susa fixation A
Susa fixation B
Synthetic Complete (SC) medium
Synthetic Complete (SC) plates
T-TYN agar
T-TYN broth
TBE (10X)
TBS-Tween
TE Buffer (100:1)
TE Buffer (10:1)
Tetracycline (10 mg/ml)
Thiamine (1%)
Trichloroacetic Acid (TCA 100%)
Tricine (1 M)
Triethanolamine (1 M)
Tris-HCl (1M)
Tris-HCl (2M)
Tris-acetate-EDTA buffer (50x TAE)
Tris-buffered saline I (TBS, 1X)
Tris-buffered saline II (TBS, 1X)
Tris-glycine Native-Polyacrylamide Gel loading buffer (2X)
Tris-glycine Native-Polyacrylamide Gel running buffer (10X)
Tris-glycine SDS-Polyacrylamide Gel (10% Separating)
Tris-glycine SDS-Polyacrylamide Gel (12% Separating)
Tris-glycine SDS-Polyacrylamide Gel (15% Separating)
Tris-glycine SDS-Polyacrylamide Gel (18% Separating)
Tris-glycine SDS-Polyacrylamide Gel (5% Stacking)
Tris-glycine SDS-Polyacrylamide Gel (8% Separating)
Tris-glycine SDS-Polyacrylamide Gel loading buffer (2X)
Tris-glycine SDS-Polyacrylamide Gel loading buffer (3X)
Tris-glycine SDS-Polyacrylamide Gel running buffer (10X)
Trypsin/EDTA (0.025% Trypsin 0.01% EDTA)
Trypsin/EDTA (0.05% Trypsin 0.02% EDTA)

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