Primo --- Your Ultimate PCR Primer Design Tools

Primo 3.4 Online*

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Primo Pro Standard PCR, reduces primer dimer and random primering. Batch mode. Primo Multiplex Multipe PCR in one tube. Reduces dimers and random primering.
Primo Degenerate Degenerate primers based on single protein, or multiple alignments of proteins or nucleotides Primo Diverse Single pair of primers to amplify regions that are most diverse in a gene family
Primo Unique Multiple primer pairs, each uniquely amplify one gene in a family Primo MSP Methylation-specific PCR and bisulphite sequencing primers
Primo Profile Design degenerate primers to profile large number of genes Primo Random Generates random primers for RAPD, AP-PCR, and RAP-PCR.
Primo Optimum Design primers for optimizing gene expression. Gene synthesis. Primo HighLow For templates with high/low GC, or repeats, or long templates.
Primo Melt Denaturing/Temperature Gradient Gel Electrophoresis (DGGE/TGGE) primers Primo Oligo Calculator for making primer stocks and dilutions.
Primo Sequencing Primer design for DNA sequencing. Primo In Vitro Oligo / Primer design for in vitro transcript / translation.
Primo SiteD Primer design for site-directed mutagenesis. Primo DOP Primer design for DOP-PCR.
Primo Inverse Primer design for inverse PCR to clone flanking sequences. Primo SNP Primer design for SNP PCR, SBR, pyrosequencing, AS-PCR, LCR, PCR-RFLP .
Primo Find Locate primer/oligo on DNA sequences. Primo CyNew Cy3/Cy5 labeling of probe DNA.

PCR Glossary Brief introduction to PCR technologies.
Molecular Biology Glossary Brief introduction to molecular biology technologies.
*Online versions may have limited functions and may not support certain web browsers. Internet Explorer is preferred and Mac OSX users may experience problems (If having problems, switch to the classical environment for Primo online). Stand-alone versions, which are not dependent on web browsers, are always suggested.
*Tell us what you think. Comments and suggestions are always appreciated.

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