Hemocytometer Calculator

Hemocytometer (Hematocytometer, Hemacytometer) or Cell Counting Chamber is frequently used to count cell numbers such as blood count and sperm count in clinical labs, and cell density of cultured cells in research labs. The procedure is simple: cells in a single-cell suspension are mixed with a dye, usually Trypan Blue (0.8 mM in PBS) to mark dead cells. Usually 10-15 ml cells are mixed with equal volume of Trypan Blue and allow to sit in the room temperature for a few minutes but less than 20 minutes. Viable cells exclude Trypan Blue, while dead cells stain blue due to Trypan Blue uptake. Cells are loaded onto a hemocytometer (a counting chamber covered with a cover slide) and then counted under a microscope.

In general more than one hundred total cells should be counted to minimize random errors. For cells on the boundaries, only cells intersect two of the boundaries are counted. For beginners, please click on the "Training" button to learn how to count.

To start using the calculator, click on "Clear", and input your count numbers, and then click on "Go."

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