Henderson-Hasselbalch Calculator
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The Electronic Protocol Book Table of contents
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Henderson-Hasselbach equation
pH = pKa + log [A-]/[HA]
or
pH = pKa + log [salt]/[acid]
pKa for commonly used buffers
| Name | pKa | Acids/Bases | Temperature | Notes
| | ACES | 6.9 | Acid | 20 | 2-[(2-amino-2-oxoethyl)amino]ethanesulphonic acid
| | Acetic | 4.75 | Acid | 25 |
| | ADA | 6.6 | Acid | 20 | N-(2-acetamido)-2-iminodiacetic acid
| | Ammonia | 9.25 | Base | 25 |
| | Aniline | 4.63 | Base | 25 |
| | Benzoic | 4.2 | Acid | 25 |
| | Benzylamine | 9.33 | Base | 25 |
| | Bicine | 8.35 | Acid | 20 | n,n-bis(2-hydroxyethyl) glycine
| | BIS-TRIS Propane | 6.8 | Acid | 20 | 1 ,3-bis[tris(hydroxymethyl)methylamino] propane
| | Borate (1) | 9.14 | Acid | 20 | o-Boric
| | Borate (-2) | 12.74 | Acid | 20 | o-Boric
| | Borate (-3) | 13.8 | Acid | 20 | o-Boric
| | CAPS | 10.4 | Acid | 20 | 3-(cyclohexylamino) propanesulphonic acid
| | Carbonic (1) | 6.37 | Acid | 25 |
| | Carbonic (-2) | 10.25 | Acid | 25 |
| | CHES | 9.5 | Acid | 20 | 2- (cyclohexylamino) ethanesulphonic acid
| | Citrate (1) | 3.13 | Acid | 25 |
| | Citrate (-2) | 4.76 | Acid | 25 |
| | Citrate (-3) | 6.4 | Acid | 25 |
| | Diethylamine | 11.09 | Base | 20 |
| | Diethylmalonic | 7.2 | Acid | - |
| | Dimethylamine | 10.73 | Base | 25 |
| | Diphenylamine | 0.79 | Base | 25 |
| | Ethylamine | 10.81 | Base | 20 |
| | Ethylenediamine (1) | 10.08 | Base | 20 |
| | Ethylenediamine (-2) | 6.99 | Base | 20 |
| | Formic | 3.75 | Acid | 20 |
| | Glycylglycine | 8.4 | Acid | 20 |
| | HEPES | 7.55 | Acid | 20 | N-2-hydroxyethylpiperazine-N'-2-ethane-sulphonic acid
| | HEPPS | 8 | Acid | 20 | N-2-hydroxycthylpipcrazine-N'-3-propane-sulphonic acid
| | Imidazole | 7 | Acid | 20 |
| | MES | 6.15 | Acid | 20 | 2-(N-morpholino) ethanesulphonic acid
| | Methylamine | 10.66 | Base | 25 |
| | MOPS | 7.2 | Acid | 20 | 3-(N-morpholino) propanesulphonic acid
| | Morpholine | 8.33 | Base | 25 |
| | n-Butylamine | 10.78 | Base | 20 |
| | N-Methylaniline | 4.85 | Base | 25 |
| | o-Boric (1) | 9.14 | Acid | 20 | Borate
| | o-Boric (-2) | 12.74 | Acid | 20 | Borate
| | o-Boric (-3) | 13.8 | Acid | 20 | Borate
| | o-Phosphoric (1) | 2.12 | Acid | 25 | Phosphate (1)
| | o-Phosphoric (-2) | 7.21 | Acid | 25 | Phosphate (-2)
| | o-Phosphoric (-2) | 5.57 | Acid | - |
| | o-Phosphoric (-3) | 12.67 | Acid | 25 | Phosphate (-3)
| | Phenol | 9.99 | Acid | 20 |
| | PIPES | 6.8 | Acid | 20 | piperazine-N-N’-bis (2-ethanesulphonic acid)
| | Phosphate (1) | 2.12 | Acid | - |
| | Phosphate (-2) | 7.21 | Acid | - |
| | Phosphate (-3) | 12.67 | Acid | - |
| | Pyridine | 5.21 | Base | 25 |
| | Succinic (1) | 4.19 | Acid | - |
| | TES | 7.5 | Acid | 20 | 2-[tris (hydroxymothyl) methyl] amino ethanesulphonic acid
| | Tricine | 8.15 | Acid | 20 | N-[tris (hydroxymethyl) methy] glycine
| | Triethylamine | 11.01 | Base | 18 |
| | Trimethylamine | 9.81 | Base | 25 |
| | TRIS | 8.3 | Acid | 20 | tris (hydroxylmethyl) aminomethane
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