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BioDataFit 1.02: Data Fit For Biologists           Order   Home
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BioDataFit is a data fitting program specifically designed for biologists. It can be used to model dose-response, ligand-binding, enzyme kinetics, and growth inhibition. Emphasis is given to the four-parameter model or sigmoidal model, which is frequently used to calculate EC50 (IC50, DC50, or GI50) values in dose-response experiments such as drug screening and inhibition assays.
Model
Application
Four Parameter
Sigmoidal
Dose-response, drug screening, ligand binding, allosteric enzyme activity, and inhibition assays.
a x / ( x + b ) Enzyme kinetics, Vmax, and KM.
Linear Standard curves.
a exp( -b x ) + c Activity decay.
Usage To start, input your data in 2 (x and y) or 3 columns (x, y, and standard deviation of subpopulation of y). Chose a suitable model and click on "Build Model." Refine the model by removing outliers. To remove an outlier, check the "Mark Outlier" checkbox and then click on the outlier data point.
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BioDataFit Online runs on Java-enabled browsers. PC: Internet Explorer 5.5 or higher and Netscape 4.08 or higher. Mac: Internet Explorer 5.0, IE5.1.4 or higher (IE5.1 will not work because of a bug.)
The Electronic Protocol Book Table of contents
     An electronic protocol book with 500 protocols and 100 recipes. A great quick and practical reference for bench scientists as well as for new students. (Available for both PC and Mac.)   Get a copy
Glossary
IC Inhibitory concentration. IC50 --- the concentration at which growth or activity is inhibited by 50%. Applies to ligand and growth inhibition.
ED or EC Effective dose (or concentration).
LogIC or LogEC IC or EC in log10 scale. Log10 scale is frequently used when x values are a serial dilution. Better estimate of the standard error is obtained when log10 scale is used.
LD Lethal dose. LD50 --- the dose at which 50% of test subjects die.
GI Growth inhibition. GI50 --- the drug concentration giving a 50% reduction in the net protein increase.
LC50 for drugs with cytotoxic effect, is the concentration of drug where 50% of cells die (a 50% reduction in the measured protein at the end of the drug treatment as compared to that at the begining).
TGI Total growth inhibition.
Vmax Maximum reaction rate.
KM Michaelis-Menten Constant. Michaelis-Menten equation for enzyme kinetics:
V0 = VMax [S] / ( KM + [S] ).
High-throughput BioDataFit
BioDataFit HTP 1.2
Supports Wins/NT, Mac OSX, Linus, and Unix
  • Designed for high-throughput drug screens.
  • IC50 calculation for high-throughput inhibition assays.
  • Km or Vmax calculation for high-throughput enzyme kinetics assays.
  • EC50 calculation for high-throughput ligand-binding assays.
  • High-throughput linear-response assays.
  • Half-life calculation for high-throughput activity decay assays.
  • Automatically removes outliers for best results.
  • Interfaces for integrating to database and web-servers.
  • Command line for calling by scripts.
  • Save fitting curves as JPEG files.
  • Java application supports most systems.
  • Free tech support.
  • Customization to meet your needs.

    Click here for more info.


  • What is a USB Flash Disk?

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