Primo Unique 3.4: Unique For One Family Member
Primo Unique finds multiple primer pairs, each uniquely amplify one gene in a family. Primers designed individually for one member of the
gene family may also amplify a family member because of the sequence similarities they share. Primo Unique inputs
multiple sequences for a family, designs primers for each member, and
eliminates those primer pairs that might amplify a different family
See user instruction.
Other Primo PCR Primer Design Tools
Standard PCR, reduces primer dimmer and random primering. Batch mode.
Multipe PCR in one tube.
Degenerate primers based on single protein, or multiple alignments of proteins or nucleotides
Single pair of primers to amplify regions that are most diverse in a gene family
Multiple primer pairs, each uniquely amplify one gene in a family
Methylation-specific PCR primers
Primo Online runs on Java-enabled browsers|
PC: Internet Explorer 5.5 or higher and Netscape 4.08 or higher
Mac: Internet Explorer 5.0, IE5.1.4 or higher (IE5.1 will not work because of a bug.)
Need to raise a better antibody? |
Please use online Abie Pro 3.0 for
peptide antibody design.
Compare Primo to other online primer design software and find
Oligo synthesis facilities
The Electronic Protocol Book Table of contents
An electronic protocol book with 500 protocols and
100 recipes. A great quick and practical reference for bench scientists
as well as for new students. (Available for both PC and Mac.)
Get a copy
BioToolKit 300 (PC and Mac)   Order download
Primo 3.4 (PCR primer design) and Abie 3.0 (peptide antibody design)
are parts of functions included in BioToolKit 300 , a
collection of tools frequently used by bench biomedical scientists:
Primo 3.4 PCR primer design: |
Pro --- Standard PCR and RT-PCR.
Multiplex --- Multiple PCRs in one tube.
Degenerate --- Degenerate PCR for one or more genes.
Optimum --- Optimize codon usage. Gene Synthesis.
Diverse --- Most diverse regions of a family.
Unique --- Amplify one gene from a family.
MSP --- Methylation specific PCR.
Random --- Random primers for fingerprinting.
Profile --- Profile 10s-1000s of genes.
Abie Pro 3.0: Select antigenic peptide for antibody production and reduce background cross-reactions.
Centrifugation speed/force conversion for any rotor.
Molecular weight calculation for oligos, peptides, and chemicals.
Molar calculator for concentration-related calculations.
Spectrometer calculator for DNA and protein concentration.
Stock recipe calculator for tens of commonly used stock solutions.
Units conversion in 14 categories.
Master cocktail mix calculator for multiple reactions.
Clinical: time/date conversion, body-mass index, body area.
MicroHelper: Merge, filter, transform, and normalize microarray data.
Godlist Manager: Batch GenBank search for info or sequence. Generate web links to databases.
Heatmap Viewer: Display high-throughput data using colors. Save image as JPEG file for presentation.
LabLabel: Label and barcode printing
LabInventory: Practical inventory database
PrimerBase: Organize primers.
Searchable by name and sequence.
PeptideBase: Database for peptides.
GradeBook: Grade book for professor/TA
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