Primo Optimum 3.4: Gene Synthesis and Optimization For Gene Expression
Primo Online runs on Java-enabled browsers|
PC: Internet Explorer 5.5 or higher and Netscape 4.08 or higher
Mac: Internet Explorer 5.0, IE5.1.4 or higher (IE5.1 will not work because of a bug.)
Need to raise a better antibody? |
Please use online Abie Pro 3.0 for
peptide antibody design.
Optimizing translational signals:
Sequences surrounding the AUG translational initiation site are not random.
Eukaryotic translational initiation site has the consensus Kozak sequences.
The Shine Dalgarno sequence in bacteria promotes translation initiation by
promoting ribosome binding. Translational signals are species-specific.
Optimizing translational signals to match that of the host species is strongly
suggested for improving protein expression in the host.
Translational initiation sequences for hundreds of species including vertebrate,
invertebrate, plants, and bacteria have been extracted from the GenBank database.
Click here for more information.
Primo Optimum is designed for optimal expression of genes of interest in a
host organism (e.g., E. Coli, yeast, plants, or mammalian cell lines).
Different species have different codon usage frequencies. For high level expression
in a host, optimization of the gene sequence to match the host's codon bias increases yield
and is frequently necessary. Primo Optimum provides a tool for optimizing gene expression
in any host species and designs primers for the PCR synthesis of the optimal gene.
In addition it checks for a number of factors that might be detrimental for gene
expression. User instructions.
For more PCR primer design tools (standard PCR, multiplex PCR, profile PCR, MSP PCR, degenerate PCR, etc.), please visit
THIS SOFTWARE IS PROVIDED BY CHANG BIOSCIENCE ``AS IS'' AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CHANG BIOSCIENCE OR ITS SPONSORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.
Copyright © 2002-2004 Chang Bioscience, Inc. All rights