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Primo Multiplex 3.4: Multiplex PCR Primer Design           User Instructions FAQ Order Home
Click here for more Primo family of PCR primer design tools.

   Multiplex PCR amplifies multiple DNA fragments in one PCR reaction. One frequent problem is that although individual PCR works well, when all in one tube, they don't. One of the reasons is that primers form primer-primer dimers. As the number of primers increase in a multiplex PCR, the possibility of dimers increases exponentially. Subtle differences in primer melting temperature and base composition may results in bigger differences in amplification efficiency when primers are competing for enzyme and template.   Primo Multiplex searches for sets of primers that are consistent in characteristics and don't form dimers for any combination of the primers. See user instruction.
   For normal PCR primer design, please use Primo Pro 3.4 which reduces PCR noise by lowering probability of random primering.

Order Primo Pro 3.4
Order Primo Multiplex 3.4
Primo Online runs on Java-enabled browsers
PC: Internet Explorer 5.5 or higher and Netscape 4.08 or higher
Mac: Internet Explorer 5.0, IE5.1.4 or higher (IE5.1 will not work because of a bug.)
Need to raise a better antibody?
Please use online Abie Pro 3.0 for
peptide antibody design.

Compare Primo to other online primer design software and find the advantages:
CODEHOP
DoPrimer
GeneFisher
GPRIME
NetPrimer
Oligos-U-Like
Primer3
Primer Design
Primer Generator
Primer Selection
Primo (Recommended)
STS
Web Primer (Recommended)
Web Primers
Oligo synthesis facilities
Allele Biotech
Alpha DNA
Alta Bioscience
BIOzymTC
BMS Korea
Cornell University
DNAagency
Duke University
Elim Biopharmaceuticals
Fred Hutchinson Cancer Res Center
Harvard University
Johns Hopkins University School of Medicine
Joslin Diabetes Center
MaGill University
Marshall University
Medical University of South Carolina
North Carolina State University
Ohio State University
Penn State University
Queen's University
Retrogen
Salantes Japan
Silantes GmbH
Stanford University
Thermo Electron Corporation
Tufts Medical School
UC San Francisco
UMDNJ
University of Calgary
University of Illinois at Chicago
University of Maryland
University of Minnesota Minneapolis
University of New Mexico
University of Otago
UNMC Eppley
Vanderbilt University
Virginia Commonwealth University
Wake Forest University
Yale University
The Electronic Protocol Book Table of contents
     An electronic protocol book with 500 protocols and 100 recipes. A great quick and practical reference for bench scientists as well as for new students. (Available for both PC and Mac.)   Get a copy
BioToolKit 300 (PC and Mac)   Order download
Primo 3.4 (PCR primer design) and Abie 3.0 (peptide antibody design) are parts of functions included in BioToolKit 300 , a collection of tools frequently used by bench biomedical scientists:
  • Primo 3.4 PCR primer design:
         Pro --- Standard PCR and RT-PCR.
         Multiplex --- Multiple PCRs in one tube.
         Degenerate --- Degenerate PCR for one or more genes.
         Optimum --- Optimize codon usage. Gene Synthesis.
         Diverse --- Most diverse regions of a family.
         Unique --- Amplify one gene from a family.
         MSP --- Methylation specific PCR.
         Random --- Random primers for fingerprinting.
         Profile --- Profile 10s-1000s of genes.
  • Abie Pro 3.0: Select antigenic peptide for antibody production and reduce background cross-reactions.
  • Centrifugation speed/force conversion for any rotor.
  • Molecular weight calculation for oligos, peptides, and chemicals.
  • Molar calculator for concentration-related calculations.
  • Spectrometer calculator for DNA and protein concentration.
  • Stock recipe calculator for tens of commonly used stock solutions.
  • Units conversion in 14 categories.
  • Master cocktail mix calculator for multiple reactions.
  • Clinical: time/date conversion, body-mass index, body area.
  • MicroHelper: Merge, filter, transform, and normalize microarray data.
  • Godlist Manager: Batch GenBank search for info or sequence. Generate web links to databases.
  • Heatmap Viewer: Display high-throughput data using colors. Save image as JPEG file for presentation.
  • LabLabel: Label and barcode printing
  • LabInventory: Practical inventory database
  • PrimerBase: Organize primers. Searchable by name and sequence.
  • PeptideBase: Database for peptides.
  • GradeBook: Grade book for professor/TA


  • THIS SOFTWARE IS PROVIDED BY CHANG BIOSCIENCE ``AS IS'' AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CHANG BIOSCIENCE OR ITS SPONSORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.


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