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Degenerate Oligonucleotid Primer (DOP) PCR Design           Click here for more PCR primer design tools.
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Primo DOP 3.4 --- Primer Design for Degenerate Oligonucleotid Primer (DOP) PCR

How to use Primo DOP 3.4(beta-testing)

DOP-PCR primers usually are 6 nuceotide long random sequences flanked by 5' and 3' anchor sequences. The specificity of the primers are determined by their 3' sequences.

Primo DOP 3.4 selects primers that satisfying the following criteria:

  • No primer-primer dimers.
  • Few repeats.
  • User selected length and GC contents.

    To start, simply select desired paramters and click on "Go".

    The estimated number of products are predicted number of PCR products ( < 2 kb) for a whole genome.

    For more PCR primer design tools, please visit Primo Home.

    Thanks for using Primo DOP 3.4! Please let us know your suggestions and comments.

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