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Primo Pro 3.4: PCR Primer Design           User Instructions FAQ Order Home
Primo Family
Primo Pro Standard PCR. Primo Multiplex Multipe PCR.
Primo Degenerate Degenerate primer design. Primo Diverse Amplify diverse regions in a family
Primo Unique Amplify uniquely one gene. Primo MSP MSP, bisulphite sequencing.
Primo Profile Profile large number of genes. Primo Random RAPD, AP-PCR, and RAP-PCR.
Primo Optimum Gene synthesis, optimizing gene expression. Primo HighLow For difficult templates.
Primo Melt DGGE, TGGE. Primo Oligo Making primer stocks and dilutions.
Primo Sequencing Primer for sequencing. Primo In Vitro Primer for in vitro translation.
Order today,
get a Free USB Flash Drive!
Warning! Uncheck "Avoid background priming" if PCR template is not human cDNA. Read user manuel for details.
Primo Online runs on Java-enabled browsers
PC: Internet Explorer 5.5 or higher and Netscape 4.08 or higher
Mac: Internet Explorer 5.0, IE5.1.4 or higher (IE5.1 will not work because of a bug.)
Need to raise a better antibody?
Please use online Abie Pro 3.0 for
peptide antibody design.

The Electronic Protocol Book Table of contents
     An electronic protocol book with 500 protocols and 100 recipes. A great quick and practical reference for bench scientists as well as for new students. (Available for both PC and Mac.)   Get a copy


What is new in Primo?

Primo Pro 3.4 reduces PCR noise by lowering the probability of random primering. For example ACTACCTGCTGCTGCAC for the p53 gene looks OK because it meets criteria commonly used for primer design. This primer may have high background because the 3' 8 nucleotides appears in 4800 genes in human transcriptome. Primo Pro analyzed transcriptomes to mark sequences that are over-represented, thus allow users to select primers with few over-represented sequences at the 3'-end. As a result of reduced random priming, we expect improved amplification efficiency and cleaner products, especially for RT-PCR.

For more info about K-tuple frequency and PCR primer design please read Griffais et al. Nucleic Acids Res. 1991 July 25; 19(14): 38873891.

For more PCR primer design tools,
please visit Primo Family!


Compare Primo to other online primer design software and find the advantages:
CODEHOP
DoPrimer
GeneFisher
GPRIME
NetPrimer
Oligos-U-Like
Primer3
Primer Design
Primer Generator
Primer Selection
Primo (Recommended)
STS
Web Primer (Recommended)
Web Primers
BioToolKit 300 (PC and Mac)   Order download
Primo 3.4 (PCR primer design) and Abie 3.0 (peptide antibody design) are parts of functions included in BioToolKit 300 , a collection of tools frequently used by bench biomedical scientists:
  • Primo 3.4 PCR primer design:
         Pro --- Standard PCR and RT-PCR.
         Multiplex --- Multiple PCRs in one tube.
         Degenerate --- Degenerate PCR for one or more genes.
         Optimum --- Optimize codon usage. Gene Synthesis.
         Diverse --- Most diverse regions of a family.
         Unique --- Amplify one gene from a family.
         MSP --- Methylation specific PCR.
         Random --- Random primers for fingerprinting.
         Profile --- Profile 10s-1000s of genes.
  • Abie Pro 3.0: Select antigenic peptide for antibody production and reduce background cross-reactions.
  • Centrifugation speed/force conversion for any rotor.
  • Molecular weight calculation for oligos, peptides, and chemicals.
  • Molar calculator for concentration-related calculations.
  • Spectrometer calculator for DNA and protein concentration.
  • Stock recipe calculator for tens of commonly used stock solutions.
  • Units conversion in 14 categories.
  • Master cocktail mix calculator for multiple reactions.
  • Clinical: time/date conversion, body-mass index, body area.
  • MicroHelper: Merge, filter, transform, and normalize microarray data.
  • Godlist Manager: Batch GenBank search for info or sequence. Generate web links to databases.
  • Heatmap Viewer: Display high-throughput data using colors. Save image as JPEG file for presentation.
  • LabLabel: Label and barcode printing
  • LabInventory: Practical inventory database
  • PrimerBase: Organize primers. Searchable by name and sequence.
  • PeptideBase: Database for peptides.
  • GradeBook: Grade book for professor/TA


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