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The Electronic Protocol Book Table of contents BioToolKit 300 Download Trials
     An electronic protocol book with 500 protocols and 100 recipes. A great quick and practical reference for bench scientists as well as for new students.   Get A Copy      A collection of tools frequently used by bench biomedical scientists, ranging from centrifugation force conversion, molecular weight, OD, recipe calculators, to clinical calculators. Include all Primo 3.4, Abie 3.0, Heatmap Viewer, MicroHelper, Godlist Manager, label printing, and grade book.   More info

PCR Glossary  A-Z A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Strand Displacement Amplification (SDA)

Strand Displacement Amplification (SDA)SDA is an isothermal nucleic acid amplification method. Primer contains a restriction site is annealed to template. Amplification primers are then annealed to 5' adjacent sequences (form a nick) and start amplification at a fixed temperature. Newly synthesized DNA are nicked by a restriction enzyme, polymerase starts amplification again, displacing the newly synthesized strands. 109 copies of DNA can be made in one reaction.
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