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The Electronic Protocol Book Table of contents BioToolKit 300 Download Trials
     An electronic protocol book with 500 protocols and 100 recipes. A great quick and practical reference for bench scientists as well as for new students.   Get A Copy      A collection of tools frequently used by bench biomedical scientists, ranging from centrifugation force conversion, molecular weight, OD, recipe calculators, to clinical calculators. Include all Primo 3.4, Abie 3.0, Heatmap Viewer, MicroHelper, Godlist Manager, label printing, and grade book.   More info

PCR Glossary  A-Z A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Splicing by Overlapping Extension PCR (SOE-PCR)

Splicing by Overlapping Extension PCR (SOE-PCR)To DNA fragments are fused together by PCR without restriction digestion. 3 primer for each fragment is designed to overlap 3 sequences on the other fragment. Mix PCR products of the two fragments, and amplify using outer primers result in the production of fusion DNA. An additional step of asymmetric PCR synthesis to generate an excess of those strands which would anneal in the final PCR may be added.
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