The Electronic Protocol Book Table of contents BioToolKit 300 Download Trials
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Splicing by Overlapping Extension PCR (SOE-PCR)

Splicing by Overlapping Extension PCR (SOE-PCR)To DNA fragments are fused together by PCR without restriction digestion. 3 primer for each fragment is designed to overlap 3 sequences on the other fragment. Mix PCR products of the two fragments, and amplify using outer primers result in the production of fusion DNA. An additional step of asymmetric PCR synthesis to generate an excess of those strands which would anneal in the final PCR may be added.
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