The Electronic Protocol Book Table of contents BioToolKit 300 Download Trials
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Sequence Characterized Amplified Region (SCAR)

Sequence Characterized Amplified Region (SCAR)SCARs are DNA fragments amplified by the Polymerase Chain Reaction (PCR) using specific 15-30 bp primers, designed from nucleotide sequences established in cloned RAPD (Random Amplified Polymorphic DNA) fragments linked to a trait of interest. By using longer PCR primers, SCARs do not face the problem of low reproducibility generally encountered with RAPDs. Obtaining a codominant marker may be an additional advantage of converting RAPDs into SCARs.
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