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The Electronic Protocol Book Table of contents BioToolKit 300 Download Trials
     An electronic protocol book with 500 protocols and 100 recipes. A great quick and practical reference for bench scientists as well as for new students.   Get A Copy      A collection of tools frequently used by bench biomedical scientists, ranging from centrifugation force conversion, molecular weight, OD, recipe calculators, to clinical calculators. Include all Primo 3.4, Abie 3.0, Heatmap Viewer, MicroHelper, Godlist Manager, label printing, and grade book.   More info

PCR Glossary  A-Z A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Melting temperature

Melting temperatureAt the melting temperature half of the oligo will form duplex with complementary oligo at the same concentration. PCR annealing temperature is usually set a few degrees lower than the melting temperature.
RelatedAnnealing temperature  Nearest neighbor  
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