Primo 3.4 Online*
Click on a link to start
Standard PCR, reduces primer dimer and random primering. Batch mode.
Multipe PCR in one tube. Reduces dimers and random primering.
Degenerate primers based on single protein, or multiple alignments of proteins or nucleotides
Single pair of primers to amplify regions that are most diverse in a gene family
Multiple primer pairs, each uniquely amplify one gene in a family
Methylation-specific PCR and bisulphite sequencing primers
Design degenerate primers to profile large number of genes
Generates random primers for RAPD, AP-PCR, and RAP-PCR.
Design primers for optimizing gene expression. Gene synthesis.
||Primo HighLow For templates with high/low GC, or repeats, or long templates.
Denaturing/Temperature Gradient Gel Electrophoresis (DGGE/TGGE) primers
Calculator for making primer stocks and dilutions.
Primer design for DNA sequencing.
||Primo In Vitro
Oligo / Primer design for in vitro transcript / translation.
Primer design for site-directed mutagenesis.
Primer design for DOP-PCR.
Primer design for inverse PCR to clone flanking sequences.
Primer design for SNP PCR, SBR, pyrosequencing, AS-PCR, LCR, PCR-RFLP .
Locate primer/oligo on DNA sequences.
Cy3/Cy5 labeling of probe DNA.
PCR Glossary Brief introduction to PCR technologies.
Molecular Biology Glossary Brief introduction to molecular biology technologies.
*Online versions may have limited functions and may not support certain
web browsers. Internet Explorer is preferred and Mac OSX users may experience problems (If having problems, switch to the classical environment for Primo online). Stand-alone versions, which are not dependent on web browsers, are always suggested.
*Tell us what you think. Comments and suggestions are always appreciated.
The Electronic Protocol Book Table of contents
BioToolKit 300 Download Trials
An electronic protocol book with 500 protocols,
100 recipes, and catalog numbers for chemicals and reagents.
A great quick and practical reference for bench scientists
as well as for new students.
Get A Copy
A collection of tools frequently used by bench biomedical scientists, ranging from centrifugation
force conversion, molecular weight, OD, recipe calculators, to clinical calculators. Include all Primo 3.4, Abie 3.0, Heatmap Viewer, MicroHelper, Godlist Manager, label printing, and grade book.