Primo Cy 1.0 --- Probe Cy3/Cy6 Labeling Calculator Beta
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Primo Cy 1.0 --- Probe Cy3/Cy6 Labeling Calculator
How to use Primo Cy 1.0 --- Probe Cy3/Cy6 Labeling Calculator (beta-testing)
Primo Cy is designed for microarray direct-cDNA labeling calculations. It can also be used for the calculations of any Cy3/Cy5-labeled
Throughout the calculations, we assume the path length for all absorbance measurements is 1 cm.
Input your absorbance measurements at 260 nm, 550 nm (for Cy3 labeled probe), and 650 nm (for Cy5 labeled probe).
Input the total volume for your probes.
Input the amount of probe you would like to use in your hybridization experiments (Hyb probe amount).
There are two options:
(1) Use the same amount of probe DNA. For this option, simply enter the desired amount in pmol or mg in the "probe A260" column.
(2) Use the same number of Cy3 and Cy5 labels. For this option, enter the same desired amount in the "Cy3 A550" and "Cy5 A650" columns.
Click on "Calculate" to calculate. Results will be shown in the results window.
For microarray experiments, it is suggested the following conditions being met:
(1) Dye incorporation is greater than 15 pmol.
(2) The number of labeled nucleotides per 1000 nucleotides is greater than 20.
(3) 20 pmol of dye labels are used.
Thanks for using Primo Cy 1.0! Please let us know your suggestions and comments.
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