Online
|
Download
|
Store
|
Contact
|
About


Centrifugation Rotor Calculator           Click here for more biology tools.
Applet runs on Java-enabled browsers
PC: Internet Explorer 5.5 or higher and Netscape 4.08 or higher
Mac: Internet Explorer 5.0, IE5.1.4 or higher (IE5.1 will not work because of a bug.)
Need to design a better siRNA?
Please use SVM RNAi 3.6
for rational siRNA design.
Mini Quick Spin Centrifuge for $99! Buy Now
The Electronic Protocol Book Table of contents BioToolKit 300 Download Trials
     An electronic protocol book with 500 protocols and 100 recipes. A great quick and practical reference for bench scientists as well as for new students.   Get A Copy      A collection of tools frequently used by bench biomedical scientists, ranging from centrifugation force conversion, molecular weight, OD, recipe calculators, to clinical calculators. Include all Primo 3.4, Abie 3.0, Heatmap Viewer, MicroHelper, Godlist Manager, label printing, and grade book.   More info


Books


Centrifugation Rotor Calculator

Calculator

To convert between centrifugation speed (RPM, Revolution Per Minute) and centrifugation force (RCF, Relative Centrifugal Force):

  • 1. Choose a rotor type or input custom rotor parameters.
  • 2. Type in RPM or RCF. The other variable will be computed automatically as you type.
  • 3. k-factor is always automatically calculated.

    Rotor Comparison

    To compare rotors:

  • 1. Select two rotor types or input custom rotor parameters.
  • 2. Type in RPM or RCF for each rotor.
  • 3. To compare centrifugation time, type in a centrifugation time for one of the two rotors. Centrifugation time for the other rotor will be estimated as you type.

    Quick Guide

  • 1. Select a protocol.
  • 2. Select a rotor or input custom rotor parameters.
  • 3. RCF will be automatically set for a protocol and RMP automatically calculated.

    Note the quick guide is a general purpose guide for pelleting specific cell components. The centrifugation time is not giving here because it is dependent on specific protocol and rotor used. Please refer to your detailed protocol and use rotor comparison.

    Quick guides include the following protocols:

  • Bacteria (500-1000 g)
  • Eukaryotic cells (500-1000 g)
  • Nuclei & debris (600-1000 g)
  • Clear cell debris (10,000 g)
  • Larger subcellular particles (10,000 g) (mitochondria, chloroplasts, lysosomes, peroxisomes )
  • Smaller subcellular particles (100,000 g) (ribosomes, endoplasmic reticulum fragments, cell membranes, and microsomes)
  • Subunits (300,000 g) (ribosome subunits, polysomes)
  • Nucleic acid ethonal/isoproponal precipitation (10,000 g)
  • TCA protein precipitation (10,000 g)
  • Virus (45,000-50,000 g)
  • P100 & S100 (100,000 g)

    The following rotors are included:

    Beckman: A-100/18, A-100/30, A-110, A-95, ACR-90, An-50Ti, An-60Ti, Batch Rotor, C0650 in Allegra 21 Series, C0650 in Allegra X-22 Series, C1015 in Allegra 21 Series, C1015 in Allegra X-22 Series, CF-32Ti, EM-90, F0485, F0630 in Allegra 21 Series, F0630 in Allegra X-22 Series, F0650, F0685 in Allegra 21 Series, F0685 in Allegra X-22 Series, F0850 in Allegra 21 Series, F0850 in Allegra X-22 Series, F1010 in Allegra 21 Series, F1010 in Allegra X-22 Series, F1202, F1802, F40.25, F241.5 in Microfuge R, F241.5 in Microfuge 22R, F241.5P in Microfuge 18 or 22R, F301.5, F2402H in Allegra 21 Series, F2402H in Allegra X-22 Series, F3602, FX301.5, FX6100, GA 6, GA-10, GH-3.8, GH 3.8A, GH-4.7, H6002, JLA-8.1000, JLA-9.1000, JLA-10.500, JA-10, JA-12, JA-14, JLA-16.250, JA-17, JA-18, JA-18.1, JA-20, JA-20.1, JA-21, JA-25.15, JA-25.50, JA-30.50, JCF-Z, JE-5.0, JE-6B, JLA 8.1000, JLA 9.1000, JLA 10.500, JLA-16.250, JR-3.2, JS-3.0, JS-3.4A-1250, JS-4.0, JS-4.2, JS-4.2A, JS-4.2SM, JS-4.2SMA, JS-4.3, JS-5.0, JS-5.2, JS-5.3, JS-5.9, JS-7.5, JS-13.1, JS-24.15, JS-24.38, JV-20, MLA-80, MLA-130, MLN-80, MLS-50, NVT 65, NVT 65.2, NVT 90, NVT 100, PTS 2000, PTS DLX, PTS DLXA, S0410, S2096 in Allegra 21 Series, S2096 in Allegra X-22 Series, S241.5, S4180, S5700, SW 28, SW 28.1, SW 30, SW 30.1, SW 32 Ti, SW 32.1 Ti, SW 40 Ti, SW 41 Ti, SW 50.1, SW 55 Ti, SW 60 Ti, SW 65 Ti, SX241.5, SX4250, SX4750 in Allegra X-12 Series, SX4750 in Allegra X-15R, SX4750A in Allegra X-12 Series, SX4750A in Allegra X-15R, SX4750u in Allegra X-12 Series, SX4750u in Allegra X-15R, TA-10, TA-10-250, TA-14-50, TA-15-1.5, TH-4, TI-15, Ti-14, TLA-45, TLA-55, TLA-100, TLA-100.1, TLA-100.2, TLA-100.3, TLA-100.4, TLA-110, TLA-120.1, TLA-120.2, TLN-100, TLN-120, TLS-55, TLV-100, TS-5-1.5, TS-5.1-500, Type 100 Ti, Type 16, Type 19, Type 21, Type 25, Type 28, Type 30, Type 35, Type 40, Type 42.1, Type 42.2 Ti, Type 45 Ti, Type 50, Type 50.2 Ti, Type 50.3 Ti, Type 50.4 Ti, Type 50 Ti, Type 55.2 Ti, Type 60 Ti, Type 65, Type 70.1 Ti, Type 70 Ti, Type 75 Ti, Type 80 Ti, Type 90 Ti, VAC 50, VC 53, VTi 50, VTi 65, VTi 65.1, VTi 65.2, VTi 80, VTi 90.
    Sorvall: A-384 (inner row), A-384 (outer row), A-500, A-621, A-641, A-841, A-1256, AH-629 (17 ml), AH-629 (20 ml), AH-629 (36 ml), AH-650, A/S-400, F-12/M.18, F-16/250, F-20/micro, F-28/13, F-28/36, F-28/50, FA-MICRO (in MC-12/12V/12C), FA-MICRO (in RMC-14), GSA, GS-3, H-400, H-1000B (buckets), H-1000B (microplates), H-2000B (buckets), H-2000B (microplates), H-4000, H-6000A (buckets), H-6000A (microplates), HB-4, HB-6, HBB-6, HBB-12, HG-4L, HL-2 and HL-2B, HL-4, HS-4, LA/S-400, M (inner row), M (outer row), RP45-A, RP55-S, RP70-AT, RP80-AT2 (inner row), RP80-AT2 (outer row), RP100-AT3, RP100-AT4, RP100-VT, RP120-VT, RTH-250 (buckets), RTH-250 (microplates), RTH-750 (buckets), RTH-750 (microplates), S-20/17, S-20/20, S-20/36, S45-A, S55-S, S70-AT, S80-AT2 (outer row), S80-AT2 (inner row), S80-AT3, S100-AT3, S100-AT4, S100-AT5, S100-AT6, S120-AT2, S120-AT3, S120-VT, S150-AT, SA-300, SA-600, SE-12, SH-80, SH-3000 (buckets), SL-50RT, SL-50T, SL-250T, SLA-600TC, SLA-1000, SLA-1500, SLA-3000, SM-24 (inner row), SM-24 (outer row), SP/X, SS-34, SS-34/KSB, ST-H50, ST-H750 (buckets), ST-MICRO, STEPSAVER 50 V39, STEPSAVER 65 V13, STEPSAVER 70 V6, Surespin 630 (17 ml), Surespin 630 (36 ml), SV-288, SV-80, T-647.5, T-865, T-865.1, T-875, T-880, T-890, T-1250, T-1270, TFT-45.6 (inner row), TFT-45.6 (outer row), TFT-80.2, TFT-80.4, TH-641, TH-660, TV-1665, TV-860, TV-865, TV-865B, TZ-28, TZ-28/GK. More rotors will be added in the future. If you would like to have your favorite rotor added into this program, please feel free to send us a suggestion. Suggestions of protocols are also welcome.

    Thanks for using our software! Please let us know your suggestions and comments.

  • THIS SOFTWARE IS PROVIDED BY CHANG BIOSCIENCE ``AS IS'' AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CHANG BIOSCIENCE OR ITS SPONSORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.


    Copyright 2002-2004 Chang Bioscience, Inc. All rights reserved.