Online
|
Download
|
Store
|
Contact
|
About


Hemocytometer Cell Count Calculator           Click here for more biology tools.
Applet runs on Java-enabled browsers
PC: Internet Explorer 5.5 or higher and Netscape 4.08 or higher
Mac: Internet Explorer 5.0, IE5.1.4 or higher (IE5.1 will not work because of a bug.)
Need to design a better siRNA?
Please use SVM RNAi 3.6
for rational siRNA design.
Hemocytometer --- Cell Counting Chamber
$30 each. Order Now.
The Electronic Protocol Book Table of contents BioToolKit 300 Download Trials
     An electronic protocol book with 500 protocols and 100 recipes. A great quick and practical reference for bench scientists as well as for new students.   Get A Copy      A collection of tools frequently used by bench biomedical scientists, ranging from centrifugation force conversion, molecular weight, OD, recipe calculators, to clinical calculators. Include all Primo 3.4, Abie 3.0, Heatmap Viewer, MicroHelper, Godlist Manager, label printing, and grade book.   More info

Hemocytometer Calculator

Hemocytometer (Hematocytometer, Hemacytometer) or Cell Counting Chamber is frequently used to count cell numbers such as blood count and sperm count in clinical labs, and cell density of cultured cells in research labs. The procedure is simple: cells in a single-cell suspension are mixed with a dye, usually Trypan Blue (0.8 mM in PBS) to mark dead cells. Usually 10-15 ml cells are mixed with equal volume of Trypan Blue and allow to sit in the room temperature for a few minutes but less than 20 minutes. Viable cells exclude Trypan Blue, while dead cells stain blue due to Trypan Blue uptake. Cells are loaded onto a hemocytometer (a counting chamber covered with a cover slide) and then counted under a microscope.

In general more than one hundred total cells should be counted to minimize random errors. For cells on the boundaries, only cells intersect two of the boundaries are counted. For beginners, please click on the "Training" button to learn how to count.

To start using the calculator, click on "Clear", and input your count numbers, and then click on "Go."

Thanks for using our software! Please let us know your suggestions and comments.

THIS SOFTWARE IS PROVIDED BY CHANG BIOSCIENCE ``AS IS'' AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CHANG BIOSCIENCE OR ITS SPONSORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.


Copyright 2002-2004 Chang Bioscience, Inc. All rights reserved.