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PAGE --- recipe calculator    Click here for more online biology tools.

Tris-glycine SDS-Polyacrylamide Gel (SDS-PAGE Calculator)
Stacking Separating
Gel percentage (%) Gel percentage (%)
30% Polyacrylamide (mL) 30% Polyacrylamide (mL)
1M Tris(pH6.8)(mL) 1.5M Tris(pH8.8)(mL)
10% Ammonium persulfate (mL) 10% Ammonium persulfate (mL)
10% SDS (mL) 10% SDS (mL)
TEMED (mL) TEMED (mL)
H2O (mL) H2O (mL)
Total volume (mL) Total volume (mL)

Click here if polyacrylamide stock is 40%.
Click here for native PAGE.

What is SDS-Page?

Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis.

What is SDS-Page used for?

Separation of molecules such as proteins and DNAs.

Where to find SDS-PAGE protocols?

Click here for a collection of protocols for molecular biology.

Have you used precast gels?

Precast SDS-PAGE gels are available from vendors such as Biorad and Invitrogen.

The Electronic Protocol Book Table of contents BioToolKit 300 Download Trials
     An electronic protocol book with 500 protocols and 100 recipes. A great quick and practical reference for bench scientists as well as for new students.   Get A Copy      A collection of tools frequently used by bench biomedical scientists, ranging from centrifugation force conversion, molecular weight, OD, recipe calculators, to clinical calculators. Include all Primo 3.4, Abie 3.0, Heatmap Viewer, MicroHelper, Godlist Manager, label printing, and grade book.   More info

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