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Henderson-Hasselbalch Calculator
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Henderson-Hasselbach equation

pH = pKa + log [A-]/[HA]

or

pH = pKa + log [salt]/[acid]

pKa for commonly used buffers

Phosphate (1)
NamepKaAcids/BasesTemperatureNotes
ACES6.9Acid202-[(2-amino-2-oxoethyl)amino]ethanesulphonic acid
Acetic4.75Acid25
ADA6.6Acid20N-(2-acetamido)-2-iminodiacetic acid
Ammonia9.25Base25
Aniline4.63Base25
Benzoic4.2Acid25
Benzylamine9.33Base25
Bicine8.35Acid20n,n-bis(2-hydroxyethyl) glycine
BIS-TRIS Propane6.8Acid201 ,3-bis[tris(hydroxymethyl)methylamino] propane
Borate (1)9.14Acid20o-Boric
Borate (-2)12.74Acid20o-Boric
Borate (-3)13.8Acid20o-Boric
CAPS10.4Acid203-(cyclohexylamino) propanesulphonic acid
Carbonic (1)6.37Acid25
Carbonic (-2)10.25Acid25
CHES9.5Acid202- (cyclohexylamino) ethanesulphonic acid
Citrate (1)3.13Acid25
Citrate (-2)4.76Acid25
Citrate (-3)6.4Acid25
Diethylamine11.09Base20
Diethylmalonic7.2Acid-
Dimethylamine10.73Base25
Diphenylamine0.79Base25
Ethylamine10.81Base20
Ethylenediamine (1)10.08Base20
Ethylenediamine (-2)6.99Base20
Formic3.75Acid20
Glycylglycine8.4Acid20
HEPES7.55Acid20N-2-hydroxyethylpiperazine-N'-2-ethane-sulphonic acid
HEPPS8Acid20N-2-hydroxycthylpipcrazine-N'-3-propane-sulphonic acid
Imidazole7Acid20
MES6.15Acid202-(N-morpholino) ethanesulphonic acid
Methylamine10.66Base25
MOPS7.2Acid203-(N-morpholino) propanesulphonic acid
Morpholine8.33Base25
n-Butylamine10.78Base20
N-Methylaniline4.85Base25
o-Boric (1)9.14Acid20Borate
o-Boric (-2)12.74Acid20Borate
o-Boric (-3)13.8Acid20Borate
o-Phosphoric (1)2.12Acid25
o-Phosphoric (-2)7.21Acid25Phosphate (-2)
o-Phosphoric (-2)5.57Acid-
o-Phosphoric (-3)12.67Acid25Phosphate (-3)
Phenol9.99Acid20
PIPES6.8Acid20piperazine-N-Ní-bis (2-ethanesulphonic acid)
Phosphate (1)2.12Acid-
Phosphate (-2)7.21Acid-
Phosphate (-3)12.67Acid-
Pyridine5.21Base25
Succinic (1)4.19Acid-
TES7.5Acid202-[tris (hydroxymothyl) methyl] amino ethanesulphonic acid
Tricine8.15Acid20N-[tris (hydroxymethyl) methy] glycine
Triethylamine11.01Base18
Trimethylamine9.81Base25
TRIS8.3Acid20tris (hydroxylmethyl) aminomethane

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